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. 2015 Sep 29;10:6105–6119. doi: 10.2147/IJN.S85265

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© 2015 Xu et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Characterization of primary rat brain microvascular endothelial cells.

Notes: (A) Primary rat brain microvascular endothelial cells form a pure, near-confluent monolayer by light microscopy (upper left panel). Factor VIII (a marker for endothelium; red), ZO-1, and Claudin-5 (tight junction proteins; red) expressions are positive in primary rat brain microvascular endothelial cells by confocal microscopy. (B) Characterization of primary astrocyte cultures in different confluence culture condition by light microscopy (upper left panel) and confocal microscopy. Astrocytes are positive for GFAP.

Abbreviation: GFAP, glial fibrillary acidic protein.