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Immunofluorescent staining of tight junction protein ZO-1 (red) in confluent brain endothelial cell in triple coculture.
Notes: The ZO-1 immunostaining of endothelial cells in coculture shows a continuous, smooth, pericellular, belt-like pattern in control and PS-NP groups. However, ZO-1 immunostaining was significantly decreased in the 10 μg/mL Ag-NPS group compared to the control.
Abbreviations: Ag-NPS, silver nanoparticle suspension; PS-NP, polystyrene nanoparticle.
