Table 2.

RT-PCR validation of selected genes from microarray data

Gene	Ag-NPS		Ag ion
	RT-PCR (mean ± SE)*	Microarray (Log2)#	RT-PCR (mean ± SE)*	Microarray (Log2)#
Txnip	4.20±0.16	1.836	2.32±0.10	1.109
Ddit4	2.87±0.15	1.581	1.94±0.12	0.883
Nr4a1	0.03±0.00	−3.463	0.35±0.14	−1.982
Egr2	0.08±0.01	−2.898	0.43±0.12	−1.292
Fos	0.29±0.02	−1.462	0.97±0.13	−0.192

Notes:

^*Mean ± SE; experiment was performed in triplicate;

^#Log₂ = Log₂ (gene expression ratio of Ag-NPS or Ag ion/Control).

Abbreviations: Ag-NPS, Ag-NP suspension; RT-PCR, reverse transcriptase polymerase chain reaction; SE, standard error.