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. 2015 Sep 30;10:6075–6087. doi: 10.2147/IJN.S90887

Figure 3.

Figure 3

Mitochondrial apoptosis induced by three rod-like gold-mesoporous silica nanoparticles.

Notes: (A) MTT assay showing MCF-7 cells after incubation with three rod-like gold-mesoporous silica nanoparticles at various concentrations (1.56–200 µg/mL) for 24 hours. (B) MTT assay showing MCF-7 cells after separate incubation with 12.5 µg/mL of three rod-like gold-mesoporous silica nanoparticles for various time intervals (24, 48, or 72 hours). (C) MCF-7 cells were treated separately with 12.5 µg/mL of three rod-like gold-mesoporous silica nanoparticles for 24 hours; the apoptotic ratio was then assessed by Annexin V-FITC/PI binding and measured using flow cytometry. (D) Changes in the MMP were detected using JC-1 staining and analyzed by flow cytometry. The data represent three separate experiments. Mean values ± SD. *P<0.05 versus a control group, **P<0.01 versus a control group.

Abbreviations: Au, gold; FITC, fluorescein isothiocyanate; PI, propidium iodide; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.