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. 2015 Sep 30;10:6075–6087. doi: 10.2147/IJN.S90887

Figure 6.

Figure 6

The effect of ROS on the mitochondrial apoptosis induced by three rod-like gold-mesoporous silica nanoparticles.

Notes: MCF-7 cells were separately treated with 12.5 µg/mL of three rod-like gold-mesoporous silica nanoparticles for 24 hours in the presence or absence of 5 mM NAC. (A) Cytotoxicity was analyzed using an MTT assay. (B) The apoptotic ratio was assessed based on Annexin V-FITC/PI binding and measured using flow cytometry analysis. (C) Intracellular ROS were stained using DCFH-DA and measured using flow cytometry. (D) The expression of p-JNK and cleaved caspase-3 was analyzed using western blotting. The data represent three separate experiments. Mean values ± SD. *P<0.05 versus a control group, **P<0.01 versus a control group.

Abbreviations: Au, gold; DCFH-DA, 2′7′-dichlorofluorescein diacetate; ROS, reactive oxygen species; FITC, fluorescein isothiocyanate; PI, propidium iodide; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; NAC, N-acetylcysteine; JNK, c-Jun-N-terminal kinase; SD, standard deviation.