Table 3.

Averaged peptide penetration depth based on the AT 60/64 and 30/34 simulation series

Peptide	COM from bilayer center ± S.D. (nm)		Trp residue COM from bilayer center ± S.D. (nm)		Trp residue COM from peptide COM ± S.D. (nm)
	60/64	30/34*	60/64	30/34	60/64	30/34
WT	1.84 ± 0.16	1.78 ± 0.12	1.20 ± 0.16	1.14 ± 0.11	−0.64 ± 0.06	−0.64 ± 0.07
K8E	1.88 ± 0.24	1.89 ± 0.20	1.39 ± 0.32	1.44 ± 0.35	−0.49 ± 0.12	−0.44 ± 0.18
K15E	1.70 ± 0.15	1.71 ± 0.11	1.08 ± 0.17	1.08 ± 0.12	−0.63 ± 0.06	−0.64 ± 0.06
K20E	1.62 ± 0.15	1.70 ± 0.12	1.06 ± 0.18	1.19 ± 0.20	−0.56 ± 0.11	−0.51 ± 0.11
K22E	1.69 ± 0.14	1.71 ± 0.11	1.07 ± 0.17	1.10 ± 0.15	−0.62 ± 0.09	−0.51 ± 0.09
K25E	1.90 ± 0.17	1.81 ± 0.10	1.23 ± 0.17	1.14 ± 0.10	−0.67 ± 0.06	−0.67 ± 0.06
K28E	1.84 ± 0.16	1.82 ± 0.21	1.28 ± 0.19	1.27 ± 0.30	−0.56 ± 0.09	−0.55 ± 0.12

^*For the peptide penetration depth based on the 30/34 simulations, S.D. (nm) of the means of the fifteen simulations was as follows; WT, 0.09; K8E, 0.17; K15E, 0.08; K20E, 0.09; K22E, 0.08; K25E, 0.07 and K28E, 0.20. The depths of the mutants based on the latter set were significantly different from that of WT with p<10⁻⁷ (t-test) for K25E and with smaller p-values (i.e., greater significances) for the other mutants.