Table 3.
Validation against Phenotype: agreement of MT PCR with phenotypic testing.
| Phenotype | Organism Total (n=126) | MT PCR results | ||||||
|---|---|---|---|---|---|---|---|---|
| ESBL (Genotype) | AmpC (Genotype) | Carbapenemase (Genotype) | Nil Detected | |||||
| ESBL only | 32 | 30 CTXM-1-18 CTXM-9-8 TEM-3 SHV-1 |
0 | 1 IMP-1 |
1 | |||
| ESBL plus AmpC | 15 | 13 CTXM-1-9 CTXM-9-2 TEM-2 |
1 DHA-1 |
0 | 1 | |||
| ESBL plus carbapenemase | 12 | 12 CTXM-1-7 CTXM-9-3 TEM-2 |
0 | 12 IMP-12 |
0 | |||
| AmpC only | 27 | 10 CTXM-1-5 CTXM-9-2 TEM-2 SHV-1 |
9 CMY-9 |
0 | 8 | |||
| AmpC plus carbapenemase | 3 | 0 | 3 CMY-3 |
3 IMP-3 |
0 | |||
| Enterobacteriaceae producing carbapenemase only | 21 | 0 | 0 | 21 IMP-19 VIM-2 |
0 | |||
| Non Fermenters producing Carbapenemase only | 16 | 0 | 0 | 1 VIM-1 |
15 | |||
| % Agreement MT PCR and phenotype | Agreement value (%) | |||||||
| ESBL overall | 59 | 93.2 | ||||||
| AmpC overall | 45 | 28.8 | ||||||
| Carbapenemase | 52 | 71.1 | ||||||