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TbpA mutations do not prevent surface exposure. Whole, iron-stressed gonococcal cells were exposed to trypsin for 0, 10, 20, and 30 min, after which the reaction was stopped by the addition of aprotinin. Lanes X were not treated with trypsin. Bacteria were pelleted, subjected to SDS-PAGE, and then transferred to nitrocellulose. Western blots were probed with polyclonal TbpA antibody. Full-length TbpA is 100 kDa. Trypsin cleavage resulted in TbpA fragments of approximately 95 and 55 kDa.
