TABLE 1.
Bacterial strains used in this study
| Strain | Description | Source |
|---|---|---|
| E. coli | ||
| BL21(DE3) | fhuA2 [lon] ompT gal (λ DE3) [dcm] ΔhsdS λ DE3 = λ sBamHIo ΔEcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 Δnin5 | New England Biolabs |
| C41(DE3) | F− ompT hsdSB(rB− mB−) (rB− mB−) gal dcm (DE3) uncharacterized derivative of BL21(DE3) | Lucigen |
| Top10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 deoR araD139 Δ(ara-leu)7697 galU galK rpsL(Strr) endA1 nupG | Invitrogen |
| XL-10 Gold | endA1 glnV44 recA1 thi-1 gyrA96 relA1 lac Hte Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 Tetr F′ [proAB lacIqZΔM15 Tn10(Tetr Amyr Cmr)] | Agilent Technologies |
| N. gonorrhoeae | ||
| FA19 | Wild type | 49 |
| FA6747 | TbpA− (tbpA::mTn3cat) | 29 |
| FA6905 | TbpB− (ΔtbpB) | 36 |
| FA6815 | TbpAB− (tbpB::Ω) | 32 |
| MCV511 | L3HA(343) TbpA Lbp− (tbpA∇HA lbpB::Ω) | 23 |
| MCV512 | L3HA(343) TbpA TbpB− Lbp− (tbpA∇HA lbpB::Ω ΔtbpB) | 23 |
| MCV161 | TbpA K351A point mutation TbpB− (ΔtbpB) | This study |
| MCV162 | TbpA D355A point mutation TbpB− (ΔtbpB) | This study |
| MCV163 | TbpA D355K point mutation TbpB− (ΔtbpB) | This study |
| MCV164 | TbpA N357A point mutation TbpB− (ΔtbpB) | This study |
| MCV165 | TbpA Q358A point mutation TbpB− (ΔtbpB) | This study |
| MCV166 | TbpA K359A point mutation TbpB− (ΔtbpB) | This study |
| MCV167 | TbpA K359E point mutation TbpB− (ΔtbpB) | This study |
| MCV168 | TbpA K359R point mutation TbpB− (ΔtbpB) | This study |
| MCV169 | TbpA Q360A point mutation TbpB− (ΔtbpB) | This study |
| MCV170 | TbpA Q360E point mutation TbpB− (ΔtbpB) | This study |
| MCV171 | TbpA Q360K point mutation TbpB− (ΔtbpB) | This study |
| MCV172 | TbpA loop 3 helix deletion (T350-A361) TbpB− (ΔtbpB) | This study |
| MCV181 | TbpA K351A point mutation | This study |
| MCV182 | TbpA D355A point mutation | This study |
| MCV183 | TbpA D355K point mutation | This study |
| MCV184 | TbpA N357A point mutation | This study |
| MCV185 | TbpA Q358A point mutation | This study |
| MCV186 | TbpA K359A point mutation | This study |
| MCV187 | TbpA K359E point mutation | This study |
| MCV188 | TbpA K359R point mutation | This study |
| MCV189 | TbpA Q360A point mutation | This study |
| MCV190 | TbpA Q360E point mutation | This study |
| MCV191 | TbpA Q360K point mutation | This study |
| MCV192 | TbpA loop 3 helix deletion (T350-A361) | This study |