TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| P. damselae subsp. damselae strains | ||
| AR57 | RM-71 derivative, spontaneously rifampin-resistant mutant | 9 |
| AR78 | AR57 with in-frame deletion of dly and hlyApl genes | 9 |
| AR158 | AR57 with in-frame deletion of hlyApl and hlyAch genes | 14 |
| AR119 | AR57 with in-frame deletion of dly and hlyAch genes | 14 |
| AR89 | AR57 with in-frame deletion of dly, hlyApl, and hlyAch genes | 14 |
| AR239 | AR57 with in-frame deletion of pilD gene | 15 |
| AR252 | AR57 with in-frame deletion of pilD and tadV genes | 15 |
| AR263 | AR57 with suicide plasmid pAJR80 inserted into the cheA gene | This study |
| AR264 | AR89 with suicide plasmid pAJR80 inserted into the cheA gene | This study |
| AR272 | AR89 complemented with pAJR38 | This study |
| E. coli strains | ||
| DH5α | Cloning strain; recA | Laboratory stock |
| S17-1 λpir | recA thi pro Δhsd(R− M+)RP4:2-Tc:Mu-Km:Tn7 λpir Tpr Smr | 70 |
| Plasmids | ||
| pSpark | PCR cloning vector; Ampr | Canvax |
| pHRP309 | lacZ reporter plasmid; mob Gmr | 71 |
| pTrcHisA | His6× tag fusion expression vector; Ampr | Invitrogen |
| pTrcHisA-pPhlyP | pTrcHisA with pro-PhlyP | This study |
| pNidKan | Suicide vector, derived from pCVD442; Kmr | 72 |
| pAJR80 | pNidKan containing an internal fragment of the cheA gene | This study |
| pAJR38 | pHRP309 with the hlyApl gene from strain RM-71 | 9 |
| pAJR76 | pHRP309 with the pilD gene from strain RM-71 | 15 |