Carrier allele liaSR135G minimally alters the levels of the liaF, liaS, and liaR transcripts. (A) Growth curves of strains indicating the time points used for transcript analyses (ovals). Dotted lines, growth at ME phase (OD600, 0.5) and LE phase (OD600, 1.0). The ES phase was 2 h after the LE phase. (B) TaqMan analysis of liaF, liaS, and liaR transcripts in the parental and isoallelic mutant strains at ME, LE, and ES phases. Error bars represent SEMs, and P values were determined by the t test (unequal variance). *, P < 0.05.