FIG 7.
MCFVv is responsible for V. vulnificus mitochondrion-mediated induction of apoptosis. (A and B) HeLa cells cocultured with the indicated bacterial strains at an MOI of 10 for 60 min followed by a 30-min treatment with gentamicin to kill bacteria (90 min total) were stained with JC1 and quantified as described for Fig. 5. **, P < 0.01 compared to PBS control or indicated samples. (C) Western blot detection of cytochrome c in fractionated cell lysates (cytosol/mitochondria), prepared from cells cocultured with the indicated strains at an MOI of 10 for 90 min. CoxIV and tubulin proteins were used as fractionation markers for mitochondria and cytosol, respectively. (D and E) Western blot detection of indicated proteins from the HeLa cell lysates obtained after bacterial challenge with the indicated strains at an MOI of 10 for 90 min. Actin was used a sample recovery control. (C to E) Graphs show densitometric quantification of the band intensity for the indicated proteins. Data are representative of two independent experiments (linked by solid or dashed lines) and show intensity relative to that in cells treated with rtxA1::bla strain BS1407 (gray boxes at 1.0).