FIG 8.

Cell rounding is not inhibited by the pancaspase inhibitor z-VAD-fmk (Inh). Bright-field images showing morphology of HeLa cells intoxicated with the indicated proteins in the presence of the pancaspase inhibitor z-VAD-fmk (100 μM, 4 h) or DMSO alone. Percent cell rounding was quantitated and is presented as mean ± SD (n = 100). This experiment was repeated simultaneously with the experiment whose results are shown in Fig. 3, so controls for caspase activation did not need to be presented twice.