TABLE 1.
V. vulnificus strains, plasmids, and oligonucleotide primers used in the study
| Strain, plasmid, or oligonucleotide | Relevant characteristic or sequencea | Reference |
|---|---|---|
| Strains | ||
| BS1405 | CMCP6 ΔvvhA Rifr | 18 |
| BS1406 | BS1405 ΔrtxA1 Rifr | 18 |
| BS1407 | BS1405 rtxA1::bla Rifr | 18 |
| SNG1 | BS1405 rtxA1::mcf-bla Rifr | This study |
| SNG2 | BS1405 rtxA1::mcf(C3351A)-bla Rifr | This study |
| Plasmids | ||
| pHGJ5 | rtxA1::bla; sacB, oriR6K Cmr | 18 |
| pSA8 | mcf in fusion with bla in pHGJ5; Cmr | This study |
| pSA9 | mcf(C3351A) in fusion with bla in pHGJ5; Cmr | This study |
| Oligonucleotides (5′-3′) | ||
| 1 | GAACAGGTCGACGTTCAATGCAGAGCAAGCAGCG | This study |
| 2 | GAACAGGTCGACGCCAATGCTTCGAGATCGCTCGC | This study |
| 3 | GGTTTTGGTGGGCGTGCTGATCCACTTTCTAAAC | 26 |
a
Bacterial strains and plasmids used are either Rifr (rifampin resistant) or Cmr (chloramphenicol resistant). The enzyme sites in oligonucleotides are in bold. The underlined codon for Cys is exchanged for Ala.