FIG 1.

WTA glycosylation-dependent C3 deposition via purified anti-WTA IgG or MBL on S. aureus MRSA strain USA300. (a to d) Ethanol-killed S. aureus RN4220 mutant cells were incubated without (gray area) or with (area outlined by a black line) anti-WTA IgG (50 ng) in 20 μl of buffer, and bound IgG was detected by flow cytometric analysis. (e to l) Measurement of C3 deposition on USA300 mutant strains incubated in 10% Δspa mutant-treated human serum without (gray area) or with (area outlined by a black line) anti-WTA IgG (50 ng) in 20 μl of buffer. C3 was detected by flow cytometric analysis with specific antibodies. The method used for the preparation of Δspa mutant-treated human serum is described in Materials and Methods. The results are representative of those from three independent experiments.