TABLE 2.
Biophysical properties of treponemal Tp0750 orthologs
| Tp0750 treponemal orthologa | No. of amino acids (full length/signal sequence truncated) | Molecular size (kDa) (full length/signal sequence truncated) | pI (full length/signal sequence truncated) | % Identity (M1-Y194/M1-D223)b | Signal sequence prediction (SpI or SpII)c |
|---|---|---|---|---|---|
| T. pallidum (p) | 223/201 | 25.9/23.5 | 9.1/9.1 | 100/100 | SpI (A22-G23) |
| T. paraluiscuniculi (p) | 223/201 | 26.0/23.5 | 9.1/9.1 | 99/98 | SpI (A22-G23) |
| T. phagedenis (strain 4A) (p) | 219 | 25.6 | 5.1 | 63/55 | NS |
| T. phagedenis (strain F0421) (p) | 219 | 25.6 | 5.1 | 63/55 | NS |
| T. medium (p) | 220 | 25.6 | 6.0 | 60/54 | NS |
| T. vincentii (p) | 224/202 | 26.0/23.5 | 6.0/5.5 | 58/49 | SpI (A22-G23) |
| T. putidum (p) | 220 | 25.4 | 5.7 | 55/50 | NS |
| T. denticola (p) | 220 | 25.4 | 6.2 | 55/49 | NS |
| T. pedis (p) | 220 | 25.2 | 8.8 | 54/45 | NS |
| T. azotonutricium (np) | 172 | 18.3 | 8.9 | 26/22 | NS |
| T. bryantii (np) | 495/471 | 56.0/53.3 | 8.9/8.6 | 21/9 | SpI (A24-Q25) |
a
p, pathogen; np, nonpathogen.
b
M1-Y194, C-terminal-truncated Tp0750; M1-D223, full-length Tp0750.
c
Cleavage sites are indicated in parentheses. NS, not secreted.