FIG 2.
Mutation of Ato proteins reduces autoinduction of hyphal formation. The wild-type (SC5314), stp2Δ (SVC17), ato5Δ (HDC17), ATO1G53D (Can572), and ato5Δ+ATO5 (ATO5 complemented) strains were assayed for hyphal formation. (A) Strains were grown in YAC medium for 6 h and photographed at ×100 magnification. (B) Cells were labeled with 5-carboxytetramethylrhodamine, cocultured for 2 h with RAW267.4 macrophages, fixed, and photographed at ×60 magnification. (C) Filamentous cells were quantitated in the captured images by counting at least 150 cells per condition. Results are reported as mean values ± SD from triplicate experiments.