FIG 3.
Ato proteins are required for efficient lysis of macrophages and proliferation after phagocytosis. (A) The wild-type (SC5314), stp2Δ (SVC17), ato5Δ (HDC17), ATO1G53D (Can572), and ato5Δ+ATO5 (ATO5 complemented) strains were cocultured with RAW264.7 macrophages. Macrophage death was assessed based upon lactate dehydrogenase (LDH) release. (B) Strains were cocultured in the presence and absence of RAW264.7 macrophages for 24 h. Fungal survival was calculated as the ratio of microcolonies in the presence versus the absence of macrophages. Results are reported as mean values ± SD from triplicate experiments. **, P < 0.001.