FIG 5.

ATO gene expression is dependent upon STP2. Quantitative real-time PCR analysis of cells grown in alkalinization medium. Cells of both the wild-type (SC5314) and stp2Δ (SVC17) strains were grown in YAC (alkalinizing conditions) or the same medium with glucose added (nonalkalinizing conditions), and the transcript levels of ATO1 (A) and ATO5 (B) expression were determined. Transcript abundance was normalized to that of ACT1, and the expression in glucose was set to 1. Data are expressed as mean values ± SD from triplicate experiments.