FIG 6.

ATO overexpression alters alkalinization. (A) The wild type (THE1) and strains with ATO genes under the control of a doxycycline (Dox)-repressible promoter were incubated in YAC initially at pH 4.0 under aerated conditions at 37°C with and without Dox. (A) The pH of the cultures after 8 h is shown. The results for all overexpression strains (−Dox) are statistically different from the results for the wild-type control (P < 0.001). (B to C) Ammonia released by C. albicans cells during alkalinization on solid medium in the absence of Dox after 24 h (B) or 72 h (C). Data are expressed as mean values ± SD from triplicate experiments.