Table 1.
Secreted proteins discussed in this mini-review, which were identified by a proteomic approach from both in vitro and in planta studies, and which may be involved in the B. cinerea–host interaction.
| Name | Description | Gene namea | SignalPb | Reference |
|---|---|---|---|---|
| Cell wall-degrading enzymes | ||||
| Pectinesterases | They de-esterify pectins and facilitate the subsequent action of the polygalacturonases (PGs) and pectate lyases. | BC1G_11144.1 BC1G_06840.1 BC1G_00617.1 |
Yes No Yes |
Shah et al., 2009a,b, 2012; Espino et al., 2010; Fernandez-Acero et al., 2010; González-Fernández et al., 2014 |
| Endo-PGs | They hydrolyze the internal (1–4) linkage between D-galacturonic acid units of pectin. | BC1G_11143.1 BC1G_13137.1 BC1G_13367.1 BC1G_04246.1 BC1G_02003.1 |
Yes Yes Yes Yes Yes |
Shah et al., 2009a,b, 2012; Espino et al., 2010; Fernandez-Acero et al., 2010; González-Fernández et al., 2014 |
| Pectin lyases | They cleave polygalacturonic acid into oligogalacturonides. | BC1G_07527.1 BC1G_12017.1 BC1G_12517.1 |
Yes Yes Yes |
Shah et al., 2009a,b, 2012; Espino et al., 2010; Fernandez-Acero et al., 2010; González-Fernández et al., 2014 |
| Proteases | ||||
| BcAP8 | It involves in proteolysis | BC1G_03070.1 | Yes | Shah et al., 2009a,b; Espino et al., 2010; Fernandez-Acero et al., 2010; González-Fernández et al., 2014 |
| Oxidative burst | ||||
| Glyoxal oxidase | It involves in the production of H2O2 | BC1G_01204.1 | Yes | Shah et al., 2009b, 2012; Espino et al., 2010; González-Fernández et al., 2014 |
| Host hypersensitive response (HR) | ||||
| BcPsl1 | Elicitor that induces the HR in plants | BC1G_02163.1 | Yes | Shah et al., 2009a,b, 2012; Espino et al., 2010; González-Fernández et al., 2014 |
| Carbohydrate metabolism | ||||
| Glucan β-1,3-glucosidase | It involves in the metabolism of β-1,3-glucans | BC1G_11898.1 | Yes | Shah et al., 2009b; Espino et al., 2010; González-Fernández et al., 2014 |
| β-1,3-endoglucanase | It involves in the metabolism of β-1,3-glucans | BC1G_07319.1 BC1G_02551.1 BC1G_00594.1 BC1G_09079.1 |
Yes Yes Yes Yes |
Shah et al., 2009a,b; Espino et al., 2010 |
| Exo-β-1,3-glucanase | It involves in the metabolism of β-1,3-glucans | BC1G_01033.1 BC1G_02731.1 |
Yes Yes |
Shah et al., 2009b; Espino et al., 2010 |
aGene name of the different protein isoforms for the B05.10 strain (Botrytis cinerea sequencing project, Broad Institute), which were identified through in vitro experiments by a proteomic approach.
bSignal peptide prediction calculated by using the SignalP server (http://www.cbs.dtu.dk/services/SignalP/), indicating the identification (Yes) or not (No) of signal peptide.