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. 2015 Jul 31;6(3):716–731. doi: 10.3390/insects6030716

Figure 4.

Figure 4

RT-PCR analysis of Spodoptera exigua larvae infected with WT, mutant and repair viruses. Larvae were mock-infected (1) or infected with G25 WT (2), SeBac10 WT (3), Δegt-ORF (4), Δegt-ATG (5) or egt-repair (6) viruses and processed for RT-PCR analysis at two dpi. For each PCR a water control (7) was included. The presence or absence of SeMNPV egt gene transcripts was checked by RT-PCR (upper panels), using two different primer pairs, one pair annealing within the egt ORF (A) and the second pair annealing to the 5' upstream region of egt and within the egt ORF (B). Expression of the SeMNPV ie1 gene (C) was used as infection control and expression of the S. exigua eIF5A gene (D) was used as cDNA quality control. For each RT sample, a duplo sample without RT step (non-RT) was performed in parallel (lower panels). The 2-Log DNA Ladder (0.1–10.0 kb, New England BioLabs Inc., Leiden, the Netherlands) was used in the agarose gel to estimate PCR fragment sizes.