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. 2015 Aug 28;6(3):863–888. doi: 10.3390/jfb6030863

Table 4.

Reconstruction of ocular surface using cultured immature dental pulp stem cells, hair follicle-derived stem cells, umbilical cord stem cells, and orbital fat-derived stem cells.

Author, Year, (Reference) Cell Source Methods LSCD Model Follow-up Time Evaluation Results
Monteiro et al. 2009 [50] IDPSCs; Human Cultured on AM carrier; Transplanted (n = 5);
Control: transplanted with only AM (n = 5)		 Rabbits; Chemical burn of the cornea 3 months Slit lamp evaluation; CLCM; IF; RT-PCR Reconstruction in 100% (5/5) of animals;
Gradual improvement in corneal transparency in 100% (5/5) of animals during follow-up time of 3 months;
Neovascularization: no data;
No complications
Gomes et al. 2010 [49] IDPSCs; Human Cultured on AM carrier; MCB (n = 5), SCB (n = 4); Transplanted and covered with AM;
Control: transplanted with only AM (n = 6)		 Rabbits; Filter paper saturated with 0.5 M NaOH for 25 s (MCB), and for 45 s (SCB) 3 months Slit lamp evaluation; EM; Histology; IH Reconstruction in 100% (5/5) of animals; Less organized and loose corneal epithelium in 75% (3/4) of SCB animals;
Improved corneal clarity in 100% (5/5) of MCB animals;
Superficial neovascularization in one animal
No complications
Meyer-Blazejewska et al. 2011 [48] HFSCs; Mouse Cultured on fibrin carrier; Transplanted (n = 31);
Control: no transplantation (n = 31)		 Mice; Cornea and limbus removed 5 weeks Slit lamp evaluation; Histology; IF Reconstruction in 87.5% (7/8) of animals after two weeks
Improved corneal clarity;
Neovascularization in 12.5% (1/8) of animals;
No complications
Reza et al. 2011 [52] UCSCs; Human Cultured on AM carrier; Three groups:
  • 1)

    transplanted cell sheets on AM (n = 6);

  • 2)

    transplanted with only AM;

  • 3)

    no transplantation

 Rabbits; Cornea and limbus removed 4 weeks Slit lamp evaluation; Histology; IC; IH; RT-PCR Reconstruction in 66.7% (4/6) of animals;
Corneal clarity: no data;
Severe neovascularization in one eye;
Mild superficial inflammation in one other
Lin et al. 2013 [51] OFSCs; Human Cultured in MesenPro medium;
Topical application of cells (n = 9),
Intra-limbal injection of cells (n = 3);
Control: Topical application of PBS (n = 6), Injection of PBS (n = 3), no treatment (n = 3)		 Mice; Filter paper saturated with 0.5 N NaOH onto cornea 1 week Digital camera; Histology; IH; IF; WB Reconstruction of corneal epithelium after 1 week;
Improved corneal clarity;
No neovascularization;
No complications

AM, amniotic membrane; CLCM, Confocal laser corneal microscopy; EM, electron microscopy; HFSCs, hair follicle-derived stem cells; IC, immunocytochemistry; IF, immunofluorescence; IH, immunohistochemistry; IDPSCs, immature dental pulp stem cells; LSC, limbal stem cells; LSCD, limbal stem cell deficiency; MCB, mild chemical burn; OFSCs, orbital fat-derived stem cells; PBS, phosphate buffered saline; RT-PCR, reverse transcriptase polymerase chain reaction; SC, stem cell; SCB, severe chemical burn; UCSCs, umbilical cord stem cells.