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. 2015 Sep 16;6(3):946–962. doi: 10.3390/jfb6030946

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Physical appearance of membranes prepared from either fibroin alone (A, D, and G), tropoelastin-fibroin blend (10:90 ratio) (B, E, and H), and layered solutions of fibroin and tropoelastin (C, F, and I); (A–C): gross appearance of each membrane when placed over printed text (16-mm diameter discs); (D–F): internal structures revealed by scanning electron microscopy following freeze-fracture; and (G–I) visualization of tropoelastin (green) by immunolabelling and confocal fluorescence microscopy (the presence of fibroin revealed as blue autofluorescence).