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. 2015 Oct 9;5:14809. doi: 10.1038/srep14809

Figure 2. Aβ42 pentamers and hexamers are artifacts of SDS-PAGE analysis.

Figure 2

GdnHSCN-SEC analysis of (a) LMW Aβ40, (b) LMW Aβ42, (c) LMW_CL Aβ40, and (d) LMW_CL Aβ42. ESI-MS spectra corresponding to peaks detected after GdnHSCN-SEC analysis of (e) LMW_CL Aβ40 and (f) LMW_CL Aβ42 samples. SDS-PAGE analysis of isolated CL dimers and trimers, as well as mixtures of them for (g) Aβ40 and (h) Aβ42 obtained after GdnHSCN-SEC fractionation. M = monomers, D = dimers, Tr = trimers, Te = tetramers, P = pentamers, and Hx = hexamers. The red arrows indicate oligomers formed artifactually in the presence of SDS.