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Protein synthesis using ligation chemistries. (a) Native Chemical Ligation (NCL) enables the selective reaction of protein or peptide thioesters with N-terminal cysteine residues, allowing for the generation of native peptides bonds under physiological conditions and without any protecting groups. (b) Inteins can be exploited to generate recombinant protein thioesters that can be used in NCL reactions, which has been termed Expressed Protein Ligation (EPL).
