Table 1.
Interaction partners, independent of binding to RNA.
| Gene Name | Protein Name | SG | TL | PB | DC | Status | SF |
|---|---|---|---|---|---|---|---|
| ATXN2 | ataxin 2 | X | X | X | BG | X | |
| ATXN2L | ataxin 2-like | X | X | BG | X | ||
| C1QBP | complement component 1, q subcomponent binding protein | New | X | ||||
| DCP1B | DCP1 decapping enzyme homolog B | X | X | BG | |||
| DDX1 | DEAD (Asp-Glu-Ala-Asp) box helicase 1 | X | X | Inf | X | ||
| DDX17 | DEAD (Asp-Glu-Ala-Asp) box helicase 17 | New | X | ||||
| EDC3 | enhancer of mRNA decapping 3 homolog | X | X | BG | |||
| EDC4 | enhancer of mRNA decapping 4 | X | X | BG | |||
| EIF4ENIF1 | eukaryotic translation initiation factor 4E nuclear import factor 1 | BG | |||||
| ERH | enhancer of rudimentary homolog (Drosophila) | New | |||||
| FMR1 | fragile X mental retardation 1 | X | X | New | |||
| FXR2 | fragile X mental retardation, autosomal homolog 2 | X | New | ||||
| G3BP2 | GTPase activating protein (SH3 domain) binding protein 2 | X | BG | ||||
| GRAN1/ FAM195A | family with sequence similarity 195, member A | X*** | BG | ||||
| GRAN2/ FAM195B | family with sequence similarity 195, member B | X*** | BG | ||||
| HNRNPC | heterogeneous nuclear ribonucleoprotein C (C1/C2) | New | X | ||||
| HNRNPM | heterogeneous nuclear ribonucleoprotein M | New | X | ||||
| LARP4 | La ribonucleoprotein domain family, member 4 | New | |||||
| LSM12 | LSM12 homolog | BG | |||||
| LSM14A | LSM14A, SCD6 homolog A | X | X | X | BG | ||
| LSM14B | LSM14B, SCD6 homolog B | X | BG | X | |||
| NUFIP2 | nuclear fragile X mental retardation protein interacting protein 2 | X*** | BG | ||||
| PABPC1 | poly(A) binding protein, cytoplasmic 1 | X | X | Inf | X | ||
| PABPC3 | poly(A) binding protein, cytoplasmic 3 | X | Inf | ||||
| PABPC4 | poly(A) binding protein, cytoplasmic 4 (inducible form) | X | X | Inf | X | ||
| PATL1 | protein associated with topoisomerase II homolog 1 | X | X | BG | |||
| RTCB | RNA 2',3'-cyclic phosphate and 5'-OH ligase | New | X | ||||
| THRAP3 | thyroid hormone receptor associated protein 3 | New | X | ||||
| TIF1B | tripartite motif containing 28 | New |
Proteins identified by mass spectrometry from samples generated by immunoprecipitation of DDX6 from nuclease-treated samples. Status—status of interaction (New—interaction partner newly detected in this screen; Inf—interaction can be inferred from DDX6’s new function in DC, SG, TL, or PB; BG—interaction with DDX6 reported in Biogrid [55]). SG—stress granules. TL—translation. PB—processing bodies. DC—decapping complex. SF—splicing factor. ***—as demonstrated in this paper.