Table 3.

Equilibrium dissociation constants of A/Mem71with α2-3-sialyllactose (OS-11) and α2-6-sialyllactose (OS-21) between 288K and 298K, and the corresponding changes in thermodynamic functions as a result of complex formation as deduced from the temperature dependence of the dissociation constants.

Glycans	Temperature (K)	Kd (mM)	ΔG (kcal/mol)	ΔH (kcal/mol)	ΔS (kcal/mol·K)	TΔS (kcal/mol)
α2-3 sialyllactose (OS-11)	288	2.6	−3.44	−41.4	−0.13	−37.9
	293	6.4	−2.96	−41.4	−0.13	−38.6
	296	18.8	−2.35	−41.4	−0.13	−39.0
	298	26.3	−2.16	−41.4	−0.13	−39.2
α2-6 sialyllactose (OS-21)	288	6.8	−2.87	−32.2	−0.10	−29.2
	293	11.2	−2.63	−32.2	−0.10	−29.7
	296	23.8	−2.21	−32.2	−0.10	−30.0
	298	47.6	−1.82	−32.2	−0.10	−30.2