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. 2015 Jul 24;5(3):1671–1696. doi: 10.3390/biom5031671

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Binding of human galectin-3 to different neo-glycoproteins. Different amounts of galectin-3 were incubated on immobilized neo-glycoproteins (5 pmol per well) presenting (a) LacNAc-LacNAc (11a–i) and (b) LacDiNAc-LacNAc (12a–i) in an ELISA-type assay. Binging signals are plotted against galectin-3 concentration. The curves were fitted using Sigma Plot software.