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Competitive inhibition of galectin-3 binding to ASF with free tetrasaccharides and neo-glycoproteins. Residual binding of 5 µM galectin-3 to ASF in the presence of variable concentrations of (a) LacNAc-LacNAc (4) and LacNAc-LacNAc conjugated BSA (11j, 11k and 11g) and (b) LacDiNAc-LacNAc (5) and LacDiNAc-LacNAc conjugated BSA (12j, 12k and 12g) is shown. Unmodified BSA has no influence on the galectin-3 binding. Inhibition curves were fitted using Sigma Plot software.
