Table 1.
Glycosylation densities and resulting molecular weights of BSA based neo-glycoproteins 11a–k and 12a–k. The molecular weights (MW) of BSA based neo-glycoproteins were calculated according to the number of attached LacNAc-LacNAc (11a–k) and LacDiNAc-LacNAc (12a–k) glycans using TNBSA assay.
| Compound | Unmodified lysine residues [mol/mol BSA] | Amidated lysine residues [mol/mol BSA] | MW [kDa] | Compound | Unmodified lysine residues [mol/mol BSA] | Amidated lysine residues [mol/mol BSA] | MW [kDa] |
|---|---|---|---|---|---|---|---|
| BSA | 60.0 ± 0.027 | 0.0 | 66.4 | BSA | 60.0 ± 0.027 | 0.0 | 66.4 |
| 11a | 58.4 ± 0.016 | 1.6 | 67.9 | 12a | 58.3 ± 0.012 | 1.7 | 68.1 |
| 11b | 56.2 ± 0.005 | 3.8 | 70.0 | 12b | 57.8 ± 0.005 | 2.2 | 68.6 |
| 11c | 53.9 ± 0.013 | 6.1 | 72.2 | 12c | 54.1 ± 0.012 | 5.9 | 72.2 |
| 11d | 50.0 ± 0.040 | 10.0 | 75.9 | 12d | 51.2 ± 0.012 | 8.8 | 75.1 |
| 11e | 45.6 ± 0.015 | 14.4 | 80.0 | 12e | 45.9 ± 0.046 | 14.1 | 80.3 |
| 11f | 40.6 ± 0.027 | 19.4 | 84.7 | 12f | 38.7 ± 0.091 | 21.3 | 87.4 |
| 11g | 35.8 ± 0.079 | 24.2 | 89.3 | 12g | 35.6 ± 0.115 | 24.4 | 90.4 |
| 11h | 34.0 ± 0.009 | 26.0 | 90.9 | 12h | 35.0 ± 0.066 | 25.0 | 91.0 |
| 11i | 31.0 ± 0.130 | 29.0 | 93.8 | 12i | 32.5 ± 0.097 | 27.5 | 93.5 |
| 11j | 52.5 ± 0.012 | 7.5 | 73.5 | 12j | 52.6 ± 0.018 | 7.4 | 73.7 |
| 11k | 42.2 ± 0.084 | 17.8 | 83.2 | 12k | 42.0 ± 0.060 | 18.0 | 84.1 |