Table 2.
Molecular weight differences (∆MW) of neo-glycoproteins 11a–k and 12a–k compared to unmodified BSA. Values for retardation factor (Rf) were calculated based on electrophoretic mobility of samples resulting from SDS-PAGE analysis (Figure 1). Measured Rf values were transformed to molecular weights by linear regression (y = mx + b; y—log MW; m—slope; x—Rf; b—y-intercept). Differences between the calculated molecular weights of neo-glycoproteins and those of unmodified BSA are compared with data determined by TNBSA assay.
| Compound | TNBSA ΔMW [kDa] | SDS-PAGE ΔMW [kDa] | Compound | TNBSA ΔMW [kDa] | SDS-PAGE ΔMW [kDa] |
|---|---|---|---|---|---|
| BSA | 0.0 | 0.0 | BSA | 0.0 | 0.0 |
| 11a | 1.5 | 1.3 | 12a | 1.7 | 1.5 |
| 11b | 3.6 | 3.0 | 12b | 2.2 | 2.5 |
| 11c | 5.8 | 5.0 | 12c | 5.8 | 4.6 |
| 11d | 9.4 | 8.2 | 12d | 8.7 | 7.3 |
| 11e | 13.6 | 12.1 | 12e | 13.9 | 11.7 |
| 11f | 18.3 | 17.4 | 12f | 21.0 | 19.0 |
| 11g | 22.8 | 22.4 | 12g | 24.0 | 22.2 |
| 11h | 24.5 | 24.3 | 12h | 24.6 | 25.5 |
| 11i | 27.3 | 27.6 | 12i | 27.1 | 28.2 |
| 11j | 7.1 | 7.0 | 12j | 7.3 | 7.1 |
| 11k | 16.8 | 15.0 | 12k | 17.7 | 15.6 |