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(a) RP-LC-CID-MS/MS confirming the structure of the single Asn71-glycopeptide of nCG retained on ZIC-HILIC SPE; (b) Schematic illustration showing the population of non-retained chymotryptic N-glycopeptides, i.e., Asn71, Asn126, and Asn124 containing glycopeptides of nCG, azurocidin, and NE, respectively, using ZIC-HILIC SPE for glycopeptide enrichment. All glycopeptides were identified by CID- and ETD-MS/MS.
