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. 2015 Oct 9;5:14959. doi: 10.1038/srep14959

Figure 2. Effects of inflammatory stress and rapamycin on protein expression of insulin signaling pathway in vitro.

Figure 2

HepG2 cells, C2C12 myoblasts and 3T3-L1 adipocytes were treated with insulin prior to harvest. (a) IRS1, p-IRS1 (tyr), and p-AKT, proteins of insulin signaling, were downregulated in the TNF-α- or IL-6-treated group. The proteins involved in the mTOR/S6K pathway were upregulated in the presence of inflammatory cytokines. (b) Under inflammatory stress, rapamycin inhibited the protein expression of mTOR, p-mTOR and p-S6K, while increasing the proteins involved in insulin signaling.