Table 1. Activation of Olfr73 wildtype and mutants by specific odorants.
| Olfr73 | |||||
|---|---|---|---|---|---|
| EC50(μM) | wt | S113A | E208A | Y260F | S113A-E208A-Y260F |
| Jasmone | — | — | 66 ± 9 | — | 83 ± 11 |
| Rose Oxide | 35 ± 20 | 23 ± 24 | 10 ± 3 | — | — |
| Nootkatone | 225 ± 76 | — | 277 ± 54 | — | — |
| Metyl vanillate | 241 ± 42 | — | 347 ± 32 | — | — |
| MIEG | 131 ± 19 | — | 10 ± 5 | — | — |
| Ethyl vanillin | 35 ± 4 | 202 ± 44 | 147 ± 11 | 246 ± 20 | — |
EC50 values (μM) of jasmone, rose oxide, nootkatone, methyl vanillate, methyl isoeugenol (MIEG), and ethyl vanillin obtained from dose-response curves using the SEAP reporter assay measurement on wild-type and mutant receptors of Olfr73 as shown in Supplementary Fig. 4. Experiments were performed in triplicate and data are given as means ± standard deviation. Amino acids are indicated in one-letter code.