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. 2015 Oct 9;5:14561. doi: 10.1038/srep14561

Figure 4. Renal fibrosis and protein expressions of α-SMA, AGEs, and ATP5b in the kidneys of db/db and db/m+ mice.

Figure 4

(A-a) Masson’s trichrome stains were performed on renal tissue sections of db/db or db/m+ mice. Top panel: ×400, scale bar: 50 μm; bottom panel: ×1000, scale bar: 20 μm. The progression of renal fibrosis, which was shown in glomerular and tubulo-interstitial areas, was significantly increased in the db/db mice as compared with db/m+ mice. The arrow indicated the representative renal fibrosis area in db/db mice. The protein expressions of α-SMA (A-b) and ATP5b (B) were determined by Western blotting. The protein levels of immunoblotting were quantified by densitometry and normalized by GAPDH or β-actin levels. Data are presented as means ± SEM (n ≥ 3). *P < 0.05 db/db versus db/m+. (C) The levels of blood AGEs were determined by a mouse ELISA kit. Data are presented as means ± SEM (n = 16 for db/m+ mice and n = 13 for db/db mice). *P < 0.05, db/db versus db/m+ mice.