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. 2015 Oct 9;5:15117. doi: 10.1038/srep15117

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Copyright © 2015, Macmillan Publishers Limited

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Protein level of CaM (A) and CAS (B) were detected using CaM active enzyme continuous reaction spectrophotometry and immunoblotting, and control (0 d of C. reinhardtii 137AH) value of CAS activity was set to 100% for easy comparison. Gene transcript levels of CaM (C) and CAS (D) were detected using real-time RT-PCR after 0 h, 12 h, 24 h, and 48 h of N starvation. B, M, protein marker.