Fig. 5.

Pro-inflammatory cytokine secretion in livers of NAP-treated mice following acute ileitis induction. In order to induce acute ileitis, C57BL/6j wildtype mice were perorally infected with 100 cysts of T. gondii ME49 strain on day 0. Mice were treated either with synthetic NAP (open circles) or placebo (PLC; filled circles). Naive, uninfected, and untreated (None; open diamonds) mice served as negative controls. (A) MCP-1, (B) TNF, (C) nitric oxide, and (D) IL-6 concentrations were determined in ex vivo biopsies taken from livers at day 7 p.i. Numbers of analyzed animals are given in parenthesis. Medians (black bars) and level of significance (p-value) determined by Mann–Whitney U test are indicated. Data were pooled from three independent experiments