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. Author manuscript; available in PMC: 2015 Oct 9.
Published in final edited form as: J Biol Methods. 2015;2(3):e24. doi: 10.14440/jbm.2015.72

Figure 3. Horizontal PAGE purification effects on performance of DNA-DNA reactions.

Figure 3

A. Diagram shows a simple hybridization-mediated quenching reaction. B. Fluorescence titration curve shows purified fluorescein-DNA titrated with purified or unpurified quencher-DNA. C. Diagram shows a strand displacement reaction. D. Fluorescence data show de-quenching by displacer DNA in the case of a PAGE purified complex and a desalted complex.