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Stability of disulfide linkage in serum and sulfhydryls. The nanoconjugates were incubated in serum-containing PBS for 16h or in PBS containing 10mM glutathione (GSH) and 100μM cysteine for 4h, using PBS as a control. After incubation, samples were eluted using a Sephadex G-100 column. The ON content in the fractions was then detected by OD260.
