Fig. 5.
Effects of the treatments of glioma U251 cells with pre-miR-93 and antagomiR-93. VEGF (a) and IL-8 released protein (b, c) were quantified by Bio-plex analysis. RNA was isolated from cultures after 48 h in vitro growth and analyzed by RT-qPCR. Internal RT-qPCR control were U6 snRNA and let-7c for miR-93, RPL13A and 18S for IL-8 mRNA. Data are in all cases reported in comparison to U251 cells treated with control scrambled sequences. Results represent the average ± S.D. of at least three independent experiments. * p < 0.05; ** p < 0.01