TABLE 3.
ABC values for EGFR
Quantitation of EGFR levels using PE-labeled monovalent or bivalent Abs is shown. ABC values were derived from the geometric MFI of 5000 live events.
PE-EGFR × gp120 |
PE-EGFR |
|||||
---|---|---|---|---|---|---|
Cell line | Mean | S.E. | n | Mean | S.E. | n |
H1975 | 6.32 × 104 | 2.56 × 103 | 3 | 5.84 × 104 | 2.01 × 103 | 3 |
HCC827 | 4.01 × 105 | 1.27 × 104 | 2 | 3.48 × 105 | 1.35 × 104 | 4 |
H1650 | 1.21 × 105 | 1741a | 1 | 9.03 × 104 | 8.45 × 103 | 3 |
H3255 | 8.23 × 105 | 15,039a | 1 | 7.39 × 105 | 3.19 × 104 | 4 |
H820 | 1.11 × 105 | 1.41 × 103 | 2 | 9.25 × 104 | 5.60 × 103 | 2 |
H2935 | 1.34 × 105 | 15,428a | 1 | 8.24 × 104 | 4380a | 1 |
HCC4006 | 9.09 × 104 | 7852a | 1 | 5.05 × 104 | 1850a | 1 |
H1993 | 3.25 × 105 | 7.99 × 103 | 4 | ND | ||
H292 | 3.57 × 105 | 8.27 × 103 | 2 | ND | ||
SKMES-1 | 1.67 × 105 | 7.72 × 103 | 2 | ND | ||
SNU-5 | 1.11 × 105 | 6.96 × 103 | 3 | ND |
a S.E. for n = 1 was derived from nonlinear regression analysis of antibody concentration versus MFI. ND means not determined.