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. 2015 Aug 26;290(41):24715–24726. doi: 10.1074/jbc.M115.658005

TABLE 1.

Yeast strains used in this work

Strain Genotype Origin
BY wta MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Open Biosystems
Snf1Δa MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 snf1::HPH Ref. 25
Snf19myca MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 SNF1-9myc:LEU2 This study
Cyr1-TAPa MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 CYR1-TAP:HIS3 Open Biosystems
Snf1-myc Cyr1-TAPa MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 SNF1-9myc:LEU2 CYR1-TAP:HIS3 This study
Snf1-myc Cyr1-TAP reg1Δa MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 SNF1-9myc:LEU2 CYR1-TAP:HIS3 reg1::URA3 This study
Snf1-HA Cyr1-TAPa MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Snf1::HPH [pRS316-SNF1-HA] CYR1-TAP:HIS3 This study
Snf1-K84R-HA Cyr1-TAPa MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Snf1::HPH [pRS316-SNF1-K84R-HA] CYR1-TAP:HIS3 This study
Snf1Δ [pRS316]b MATa leu2-3,112 ura3-1 trp1-1 his3-11,15 ade2-1 can1-100 snf1::HPH [pRS316] This study
Snf1-HAb MATa leu2-3,112 ura3-1 trp1-1 his3-11,15 ade2-1 can1-100 snf1::HPH [pRS316-SNF1-HA] This study
Snf1-G53R-HAb MATa leu2-3,112 ura3-1 trp1-1 his3-11,15 ade2-1 can1-100 snf1::HPH [pRS316-SNF1-G53R-HA] This study
Snf1-K84R-HAb MATa leu2-3,112 ura3-1 trp1-1 his3-11,15 ade2-1 can1-100 snf1::HPH [pRS316-SNF1-K84R-HA] This study
Snf1-T210A-HAb MATa leu2-3,112 ura3-1 trp1-1 his3-11,15 ade2-1 can1-100 snf1::HPH [pRS316-SNF1-T210A-HA] This study
cyr1Δ pde1Δ yak1Δitsup;b MATa leu2-3,112 ura3-1 trp1-1 his3-11,15 ade2-1 can1-100 cyr1::KanMX, pde2::TRP1, yak1::LEU2 [YCplac33] Ref. 56
cyr1Δ pde1Δ yak1Δ Cyr1b MATa leu2-3,112 ura3-1 trp1-1 his3-11,15 ade2-1 can1-100 cyr1::KanMX, pde2::TRP1, yak1::LEU2 [YCplac33-CYR1] This study
mig1Δ a MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 mig1::KanMX4 Euroscarf
pde1Δpde2Δsnf1Δ [pRS313]b MATa leu2-3,112 ura3-1 trp1-92 his3-11,15 ade2-1 can1-100 pde1::TRP1 pde2::URA3 snf1::HPH [pRS313] This study
pde1Δpde2Δsnf1Δ Snf1-HAb MATa leu2-3,112 ura3-1 trp1-92 his3-11,15 ade2-1 can1-100 pde1::TRP1 pde2::URA3 snf1::HPH [pRS313-SNF1-HA] This study
pde1Δpde2Δsnf1Δ Snf1-G53R-HAb MATa leu2-3,112 ura3-1 trp1-92 his3-11,15 ade2-1 can1-100 pde1::TRP1 pde2::URA3 snf1::HPH [pRS313-SNF1-G53R-HA] This study
cyr1Δ pde1Δ yak1Δ Cyr1-4Eb MATa leu2-3,112 ura3-1 trp1-1 his3-11,15 ade2-1 can1-100 cyr1::KanMX, pde2::TRP1, yak1::LEU2 [YCplac33-CYR1S435E,S527E,T631E,S645E] This study

a Isogenic to BY4741.

b Isogenic to W303-1A.