TABLE 2.
Identified Snf1 interactors
| Interactor | Description | Previously identified | Sequence coverage | Peptides matched |
|---|---|---|---|---|
| % | ||||
| Snf1 complex | ||||
| Sip1 | One of three β subunits of the Snf1 kinase complex | Yes | 39.9 | 18 |
| Sip2 | One of three β subunits of the Snf1 kinase complex | Yes | 51.6 | 16 |
| Gal83 | One of three β subunits of the Snf1 kinase complex | Yes | 54.7 | 20 |
| Snf4 | Activating γ subunit of the AMP-activated Snf1p kinase complex | Yes | 76.4 | 24 |
| Sak1 | Upstream serine/threonine kinase for the SNF1 complex | Yes | 35.6 | 30 |
| Reg1 | Regulatory subunit of type 1 protein phosphatase Glc7p | Yes | 39.4 | 27 |
| Glucose metabolism | ||||
| Cdc19 | Pyruvate kinase | No | 48.8 | 14 |
| Hxk2 | Hexokinase isoenzyme 2 | Yes | 18.4 | 7 |
| Pfk1 | α subunit of heterooctameric phosphofructokinase | No | 38.3 | 20 |
| Pfk2 | β subunit of heterooctameric phosphofructokinase | Yes | 44.9 | 11 |
| Tdh2 | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2 | No | 79.2 | 21 |
| Tdh3 | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3 | No | 38.5 | 12 |
| Pdc1 | Major of three pyruvate decarboxylase isozymes | No | 47.6 | 14 |
| Eno2 | Enolase II | No | 78.7 | 24 |
| Ald6 | Cytosolic aldehyde dehydrogenase | No | 62.8 | 26 |
| Atp2 | β subunit of the F1 sector of mitochondrial F1F0 ATP synthase | No | 58.9 | 17 |
| Tal1 | Transaldolase, enzyme in the nonoxidative pentose phosphate pathway | No | 45.7 | 13 |
| Ribosomes and translation | ||||
| Rps8a | Protein component of the small (40S) ribosomal subunit | No | 35.5 | 5 |
| Rpl1a | Ribosomal 60S subunit protein L1A | No | 67.1 | 18 |
| Rpl3 | Ribosomal 60S subunit protein L3 | No | 53.8 | 21 |
| Rpl4a | Ribosomal 60S subunit protein L4A | No | 48.3 | 13 |
| Rpl6a | Ribosomal 60S subunit protein L6A | No | 17.1 | 2 |
| Rpl8a | Ribosomal 60S subunit protein L8A | Yes | 60.9 | 17 |
| Rpl17b | Ribosomal 60S subunit protein L17B | No | 8.7 | 1 |
| Rpp0 | Conserved ribosomal protein P0 of the ribosomal stalk | No | 43.6 | 12 |
| Eft1 | Elongation factor 2, also encoded by EFT2 | No | 37.0 | 10 |
| Eft2 | Elongation factor 2, also encoded by EFT1 | No | 62.7 | 41 |
| Tef1 | Translational elongation factor EF-1α, also encoded by TEF2 | No | 23.9 | 4 |
| Tef2 | Translational elongation factor EF-1α, also encoded by TEF1 | Yes | 63.8 | 21 |
| Sro9 | Cytoplasmic RNA-binding protein; associates with translating ribosomes | Yes | 24.7 | 7 |
| Cft1 | RNA-binding subunit of the mRNA cleavage and polyadenylation factor | No | 35.0 | 28 |
| Nip1 | eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3) | No | 22.2 | 13 |
| Stm1 | Protein required for optimal translation under nutrient stress | Yes | 42.5 | 12 |
| Amino acid biosynthesis and transport | ||||
| Lys20 | Homocitrate synthase isozyme | No | 61.2 | 20 |
| Lys21 | Homocitrate synthase isozyme | No | 47.5 | 19 |
| Cpa2 | Large subunit of carbamoyl phosphate synthetase | No | 50.1 | 37 |
| Npr1 | Protein kinase that stabilizes several plasma membrane amino acid transporters | Yes | 58.7 | 28 |
| Fatty acid synthesis | ||||
| Fas1 | β subunit of fatty acid synthetase | Yes | 47.0 | 66 |
| Fas2 | α subunit of fatty acid synthetase | No | 52.5 | 70 |
| IMP dehydrogenases | ||||
| Imd1 | Nonfunctional protein with homology to IMP dehydrogenase | No | 30.6 | 8 |
| Imd2 | Inosine monophosphate dehydrogenase | No | 45.3 | 18 |
| Imd3 | Inosine monophosphate dehydrogenase | No | 42.2 | 15 |
| Imd4 | Inosine monophosphate dehydrogenase | No | 42.8 | 16 |
| Chaperones | ||||
| Ssa2 | ATP-binding protein involved in protein folding and vacuolar import of proteins | Yes | 64.8 | 30 |
| Ssb1 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone | Yes | 49.8 | 21 |
| Ssb2 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone | Yes | 49.8 | 21 |
| Bmh1 | 14-3-3 protein, major isoform | Yes | 58.4 | 13 |
| Others | ||||
| Cyr1 | Adenylate cyclase, required for cAMP production and protein kinase A signaling | No | 19.7 | 19 |
| Cka1 | α catalytic subunit of CK2 | Yes | 31.3 | 3 |
| Cop1 | α subunit of COPI vesicle coatomer complex | No | 26.2 | 20 |