TABLE 3.

Identified phosphopeptides

Sequence	P site (s)	WT	snf1Δ	Modification(s)	WT				snf1Δ				Predicted phosphosites
					Xcorr	m/z	MH+	Charge	Xcorr	m/z	MH+	Charge	NetPhos Yeast	NetPhos K	Phosida
						Da	Da			Da	Da
17QEEQEQQIEQSSPTEANDR35	Ser28	×	×	Ser28 (phospho)	2.62	775.9883	2325.9503	3	4.73	775.9901	2325.9558	3	Ser28
17QEEQEQQIEQSSPTEANDR35	Thr30	×	×	Thr30 (phospho)	3.11	775.9844	2325.9386	3	4.21	775.9915	2325.9598	3
210KPSKDSTLSNHLADNVPSTLR230	Ser218	×	×	Ser218 (phospho)	1.77	787.3881	2360.1496	3	2.07	787.3915	2360.1599	3	Ser218
210KPSKDSTLSNHLADNVPSTLR230	Thr228		×	Thr228 (phospho)					1.52	814.0579	2440.1590	3		Thr228 (score, 0.91)
281SFLLGSTSSSSSRR294	Thr287	×	×	Thr287 (phospho), Ser291 (phospho)	2.18	816.3436	1631.6799	2	4.03	816.3461	1631.6849	2		Ser291 (score, 0.77)	Thr287
281SFLLGSTSSSSSRR294	Ser288, Ser291	×	×	Ser288 (phospho), Ser291 (phospho)	3.83	816.3447	1631.6822	2	3.97	816.3461	1631.6849	2	Ser288	Ser291 (score, 0.77)
346SEKVTPEYNENIPENSNSDNKR367	Thr350	×	×	Thr350 (phospho)	1.76	882.0539	2644.1471	3	4.13	882.0574	2644.1576	3	Thr350
368EATTPTIETPISCKPSLFR386	Thr370	×	×	Thr370 (phospho), Cys380 (carbamidomethyl)	3.02	743.3613	2228.0692	3	2.57	1114.5403	2228.0733	2
432KSVSMADLSVAAAAPNGEFTSTSNDR457	Ser435	×		Ser435 (phospho)	6.01	1353.6072	2706.2071	2					Ser435
483SRRSSIDADELDPMSPGPPSK503	Ser483, Ser497	×	×	Ser483 (phospho), Ser497 (phospho)	2.68	801.3379	2401.9991	3	2.23	801.3417	2402.0105	3	Ser483, Ser497
483SRRSSIDADELDPMSPGPPSK503	Ser497	×	×	Ser497 (phospho)	1.76	774.6774	2322.0176	3	1.51	774.6856	2322.0423	3	Ser497
513KDNESMVTAGDSNSSFVDICK533	Ser524	×	×	Ser524 (phospho), Cys532 (carbamidomethyl)	6.11	1192.4935	2383.9798	2	5.90	1192.4950	2383.9827	2			Ser524
513KDNESMVTAGDSNSSFVDICK533	Ser527	×		Ser527 (phospho), Cys532 (carbamidomethyl)	6.29	1192.4958	2383.9844	2					Ser527
629KFTSSSVNMNSPDGAQSSGLLLQDEK654	Thr631	×		Thr631 (phospho)	3.06	940.7603	2820.2662	3
629KFTSSSVNMNSPDGAQSSGLLLQDEK655	Ser645	×		Thr631 (phospho)	3.15	940.7603	2820.2662	3							Ser645
1041NMTSLTLNKAQLSSIPGELLTK1062	Thr1043, Thr1046, Ser1053		×	Thr1043 (phospho), Thr1046 (phospho), Ser1053 (phospho), Ser1054 (phospho)					2.33	670.5564	2679.2038	4		Thr1046 (score, 0.81)	Ser1053, Ser1054
1097NKLEYIPPELSQLK1110	Tyr1101, Ser1107		×						1.57	611.2991	1831.8828	3			Ser1107
1209LESITELYLSGNK1221	Ser1211		×						1.58	773.8703	1546.7333	2
1303SFISHDIDADLSDLTVLPQLK1323	Ser1306	×	×	Ser1306 (phospho)	1.80	803.0618	2407.1707	3	1.52	803.0770	2407.2165	3			Ser1306
1380FRGNDDECLLCLHDSKNQNADYGHNISR1407	Ser1394	×	×	Cys1388 (carbamidomethyl), Cys1391 (carbamidomethyl)	4.14	857.8657	3428.4411	4	2.91	857.8685	3428.4523	4			Ser1394
1656LQPEISPPTGNLAMVFTDIK1675	Ser1661	×	×	Ser1661 (phospho)	1.52	751.04	2251.10	3	1.63	835.0750	2503.2105	3	Ser1661
1739LLDAQWPEEITSVQDGCQVTDR1760	Ser1750	×		Ser1750 (phospho), Cys1755 (carbamidomethyl)	2.64	880.7224	2640.1527	3							Ser1750