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. 2015 Aug 25;290(41):24844–24857. doi: 10.1074/jbc.M115.668822

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Detergent affects CCO enzyme activity. A, schematic of BCO2 oxidative cleavage of zeaxanthin. B, HPLC chromatogram comparing the in vitro activity of murine BCO2 when performed with n-octyl β-d-thioglucopyranoside (OTG) (gray trace) or DMN (dashed trace) detergent. The black trace shows the control. Relative activities (measured as the production of 10Apo) comparing different detergents is shown in the inset. C, UV/visible absorbance spectra of the product 10Apo and substrate zeaxanthin. C84, tetraethylene glycol monooctyl ether; C86, hexaethylene glycol monooctyl ether; mAU, milli-absorbance units.