FIGURE 2.

Production and characterization of a recombinant Rx1 protein. A, purified Rx1 protein (1.5 μg) was separated by 12.5% SDS-polyacrylamide gel electrophoresis and stained with Coomassie Blue. Molecular mass standards (in kDa) are indicated. Protein identity was confirmed by trypsin digest and MALDI-TOF analysis. Shown are a circular dichroism spectrum for Rx1(1–489)^WT (B) and Rx1(1–489)^K176R (C) depicting experimental data (green dots) and the spectrum calculated using CDSSTR (gray dots).