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. 2015 Aug 25;290(41):24945–24960. doi: 10.1074/jbc.M115.672121

FIGURE 3.

FIGURE 3.

The Rx1 CC-NBARC domains bind nucleic acids in vitro. A, EMSA for Rx1(1–489)WT, Rx1(1–489)K176R, and BSA using 100 ng of φX174 virion DNA (ssDNA) or φX174 virion RF I DNA (dsDNA). For dsDNA, the top band represents relaxed circular DNA, whereas the bottom band represents supercoiled circular DNA. B–D, top panels, representative EMSA for Rx1(1–479)WT showing raw data for binding to nucleic acids. Bottom panels, quantitative EMSA analysis giving apparent affinities of Rx1(1–489)WT and Rx1(1–489)K176R for dsDNA (B), ssRNA (C), and ssDNA (D) (means ± S.E. (error bars); n = 3–6). E, quantitative EMSA showing binding of 1 μm full-length plant-expressed Strep-tagged Rx1 (Rx1-4Strep), E. coli produced Rx1(1–489)WT, or BSA to ssDNA (means ± S.E.; n = 8; bars with different letters are significantly different (p < 0.05); one-way ANOVA with post hoc Tukey's multiple comparison). F, quantitative EMSA analysis giving comparative affinities of PSiP-NB-ARC and PSiP-NB for ssDNA (means ± S.E.; n = 3).