FIGURE 6.
Rx1 preferentially binds distorted DNA topologies. A, quantitative EMSA analysis giving comparative affinities of Rx1(1–489)WT for ssDNA (ssF12), dsDNA (dsF12), branched dsDNA with two dsDNA arms (F12-ds/ds), branched dsDNA with two ssDNA arms (F12-ss/ss), and branched dsDNA with one ssDNA and one dsDNA arm (F12-ds/ss) (means ± S.E. (error bars); n = 3–4). B, quantitative EMSA analysis giving comparative affinities of Rx1(1–489)WT for ssDNA (ssP1), dsDNA (dsP1), and dsDNA with bubbles of varying sizes (means ± S.E.; n = 3–4).