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. 2015 Aug 25;290(41):24945–24960. doi: 10.1074/jbc.M115.672121

FIGURE 8.

FIGURE 8.

Binding of Rx1 protein domains to DNA in situ. A, the ratio of the long (>1.0 ns) to short (<0.5 ns) GFP lifetimes for GFP fusion constructs representing varying Rx1 subdomains produced in N. benthamiana leaves using agroinfiltration (n > 6; *, p < 0.05 compared with GFP by one-way ANOVA with post hoc Dunnett test). B, ratio of the long (>1.0 ns) to short (<0.5 ns) GFP lifetimes for Rx1-GFP full-length constructs alone and upon co-expression with virulent (CP105) and avirulent alleles (CP106) of the PVX CP (n > 6; *, p < 0.05 compared with GFP by one-way ANOVA with post hoc Dunnett test). C, ratio of the long (>1.0 ns) to short (<0.5 ns) GFP lifetimes for GFP-NLS-Rx1 and GFP-NES-Rx1 full-length constructs alone and upon co-expression with the avirulent allele of PVX, CP106 (n = 4; *, p < 0.05 compared with GFP by one-way ANOVA with post hoc Dunnett test). D, ratio of the long (>1.0 ns) to short (<0.5 ns) GFP lifetimes for Rx1-GFP full-length construct alone and upon co-expression with either the avirulent allele of PVX, CP106, or the Pto kinase and AvrPto (n = 12–20; *, p < 0.05 compared with GFP by one-way ANOVA with post hoc Dunnett test). Error bars, S.E.